Allow me to throw the first snowball. Are you using Gamborg's medium without MS salts and sucrose? I have a recipe for Nephrolepsis media that includes 1/2 MS salts, 20g/L sucrose, 0.1 mg/L of NAA and 1.0 mg/L of Kinetin. If you are using all of these you might try different concentrations of hormones to find one that works best for the species you are trying to grow.

i'm sorry for the late respond, I don't get on this forum often. Yes, I have and still into home based tissue culture. I have been experimenting on different plants, but haven't tried AV yet. I haven't heard of adding beef stock before, and doubt that it would of any good. There are several recipes around, I basically use complete fertilizer with trace elements with addition coconut water and tomato puree for my medium, and will be experimenting on other recipe soon - still need to find some chemicals to complete my ingredient list - my major setback is that Ammonium Nitrate is not available in the market (banned). The book PLANT FROM TEST TUBE is a good reference.

lette_04- I've been through several brands of salt grinders over the years. My first grinder I bought from King Arthur Flour Company was an Emsa, but it had a small chamber and was difficult to change the grind. I now use it to grind whole nigella seeds (a nice change-of-pace taste to black pepper). We had a set of small and large G'Rabbit grinders (has ears that look like a rabbit and they were almost a kitchen "pet"). Very easy to use with one hand, but we managed to break the ears on one of the small ones, and both sizes were messy. Fast forward several years.... I have a pair of OXO salt and pepper grinders I really like, and OXO has several different styles available. The ones I have are at the Amazon link below. I purchased mine at K-Mart, on sale, for about half the Amazon price. You can easily change the coarseness of the grind with your thumb (5 choices of grind). They are easy to use if you have a small hand (with a nice soft grip), they don't take up much cabinet space and are easy to refill. Because they store with the grinder up, you don't have a mess where ever you store them. -Grainlady Here is a link that might be useful: OXO Salt Grinder

Google them,there are free directions all ove the net. Kathi